[ODP-124] 口腔カンジダ症患者由来と非口腔カンジダ症由来のCandida albicansの比較研究
Oral candidiasis is an opportunistic infection caused by the commensal fungus Candida. Oral Candidiasis proliferates due to the host’s aging and weakened immune system caused by many medications, dry mouth and the use of dentures, resulting in oral pain and taste abnormalities. However, we thought that there might be differences not only on the host side but also in Candida itself. Therefore, we compared and investigated the pathogenicity of Candida albicans in patients with oral candidiasis and non-oral candidiasis. The subjects were patients who visited the Dental departments of Hokkaido University Hospital and complained of symptoms such as pain in the mouth and underwent a Candida examination. As a result, those with 10 or more colonies were judged to have oral candidiasis, while those with fewer than 10 colonies, even if detected, were judged to have non-oral candidiasis. The following four items were investigated using these stocks. (1) Measurement of extracellular secretory enzyme activities on agar, (2) Identification of C. albicans genotypes by PCR, (3) Measurement of IL-8 induced by C. albicans stimulation of oral epithelial cells by ELISA, and (4) Measurement of cell death induced by C. albicans in oral epithelial cells by LDH. The results were compared to determine the difference in pathogenicity between oral candidiasis and non-oral candidiasis patients.