[ODP22] 5. 病原性-b. 毒素・エフェクター・生理活性物質

[ODP-131] 黄色ブドウ球菌のα-ヘモリジン産生抑制による高麗人参サポニンの抗アトピー性皮膚炎作用

岩崎 唯那¹，蔦元 さくら²，今宮 里沙²，寒川 慶一³，岩尾 洋⁴，Dendi Krisna Nugraha⁵，堀口 安彦⁵，岡 真優子¹ (¹京都府大院・生命環境・食環境安全性，²京都府大・生命環境・食品安全性，³大阪市大院・医・分子病態薬理学，⁴四天王寺大・教育，⁵大阪大・微研・分子細菌)

The skin microbiome is directly associated with atopic dermatitis (AD). α-toxin, also known as α-hemolysin, produced by S. aureus positively correlates with AD severity and is a therapeutic target for the control of AD. We reported that red ginseng extracts (RGEs) suppressed phosphorylation of p70 ribosomal protein S6 kinase in macrophages and basophils in AD mice, suggesting that RGEs have an anti-inflammatory effect on the AD. In this study, we examined whether RGEs suppress the AD development by S. aureus. By using the RNA-seq technique, we analyzed the expression levels of 2,726 genes in S. aureus BAA-1717 strain that had been incubated for 3 or 6 h in the presence or absence of RGEs. RGEs reduced the expressions of 169 genes and increased the expressions of 129 genes. The mRNA levels of α-/γ-/δ-hemolysin and both saeS/R and agrC/A in the two component systems, which regulate the expression of the hemolysin genes, were decreased by RGEs. Next, we examined the effect of culture medium of S. aureus (Sa-CM) with or without RGEs against AD mice. The expression levels of Ifn-gamma and Ccl-2 mRNA were higher in ears of AD mice than in ears of control mice. These mRNA levels in ears of AD mice were further increased by Sa-CM without RGEs, but not by Sa-CM with RGEs. These results suggest that RGEs show inhibitory effects on the AD development by the infection with S. aureus.