[W3-3/ODP-032] Breastfeeding regulates acquisition of maternal oral bacteria in infant oral microbiota
Influx of maternal oral microbes is thought to play an important role in the acquisition and development of infant oral microbiota. In this study, we examined tongue swab samples collected from 448 mother-infant pairs at 4 months of age. After extraction of DNA from each sample, the full-length of 16S ribosomal RNA gene was amplified using primer 8F and 1492R and sequenced using PacBio single-molecule long-read sequencer. The bacterial composition of each sample was determined using the amplicon sequence variant approach. Although the infant oral microbiota was clearly different from the mother oral microbiota, their bacterial compositions were more similar to their own mother than to the unrelated mothers, sharing a median of 9.7% (range 0.0 to 99.3) of their relative abundance with their own mother. Especially, Veillonella dispar and Streptococcus salivarius were highly shared. In addition, the shared abundance was higher in the infants with lower body weight and lower birth weight. However, the exclusively breastfed infants had lower shared abundance than exclusively formula-fed infants. These results suggest that breastfeeding might regulate the acquisition of maternal oral bacteria and control the maturation of infant oral microbiota.