Double-strand break Site-Targeted PCR coupled with high concentration TBE-PAGE enables to detect NHEJ-mediated indel mutations at a 1-bp resolution

Faryal Ijaz

09:30 〜 09:42

[3A-101] Double-strand break Site-Targeted PCR coupled with high concentration TBE-PAGE enables to detect NHEJ-mediated indel mutations at a 1-bp resolution

*Faryal Ijaz¹, Ryota Nakazato¹, Mitsutoshi Setou², Koji Ikegami^1,2,3 (1. Hiroshima Univ. Graduate School of Biomedical and Health Sciences, Department of Anatomy and Developmental Biology , 2. Hamamatsu Univ. School of Medicine Department of Cellular and Molecular Anatomy and International Mass Imaging Center, 3. JST, PRESTO)

キーワード：Genotyping , Knock-out, NHEJ, Screening, CRISPR

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講演情報

[3A-101-07] 一般口頭発表6 (最新技術）

[3A-101] Double-strand break Site-Targeted PCR coupled with high concentration TBE-PAGE enables to detect NHEJ-mediated indel mutations at a 1-bp resolution

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