6:15 PM - 7:15 PM
[II-JCKP1-02] Establish Heparin / Collagen-REDV selective active interface on ePTFE to promote endothelialization
Objective To construct heparin / collagen-REDV selective active interface on the surface of expanded polytetrafluoroethylene, and observe the endothelialization level and cell activity on the surface of ePTFE in vitro. Methods Five layers of heparin and collagen combined coating [(HEP/COL)5] were prepared on the surface of 0.1mm ePTFE membrane by layer by layer self-assembly technique, and REDV peptides were coated on its surface ,then a selective active interface on ePTFE was obtained. Unmodified ePTFE、(HEP / COL)5 modified ePTFE、(HEP/COL)5-REDV modified ePTFE and (HEP/COL)5-REVD modified ePTFE were co-cultured with umbilical vein endothelial cells for 24 hours to 72 hours , observed the endothelialization level and cell activity on ePTFE. Results Co-culture 1h and 6h, the number of endothelial cells adherent on (HEP/COL)5-REDV modified ePTFE was more than other groups(P<0.05); Co-culture 24h、48h、72h,the endothelial cells on modified ePTFE were significantly more than unmodified , the endothelial cell number and cell activity was highest in (HEP/COL)5-REDV modified ePTFE group(P<0.05). Conclusions Using layers by layer self-assembly technique to build a selective active interface on ePTFE surface can promote endothelial cells adherent to ePTFE , as well as promote cell proliferation and improve cell activity.