[1P] 81 Imaging and Visualization

[1P-383] Development of a post hoc neuron labeling method employing immunofluorescence staining and intracellular dye injection to the fixed slice specimens.

*Shinichiro Okamoto^1,2,3, Megumu Takahashi^1,2,4, Haruhi Mizuma^1,2, Tomoyo Kobayashi^1,2, Masato Koike², Hiroyuki Hioki^1,2,5 (1. Dept Neuroanat, Grad Sch Med, Juntendo Univ, Tokyo, Japan, 2. Dept Cell Biol and Neurosci, Grad Sch Med, Juntendo Univ, Tokyo, Japan, 3. Adv Res Inst Health Sci, Juntendo Univ, Tokyo, Japan, 4. Cent Genom and Regen Med, Grad Sch Med, Juntendo Univ, Tokyo, Japan, 5. Dept Multi-Scale Brain Struct Imaging, Grad Sch Med, Juntendo Univ, Tokyo, Japan)

Keywords:post-hoc labeling, intracellular dye injection, dendrite, paraventricular hypothalamic nucleus