[1P] 81 Imaging and Visualization

[1P-380] Multi-scale, long-term in vivo two-photon imaging of neuronal structures and functions in mice utilizing novel cranial window based on fluoropolymer nanosheet

*Taiga Takahashi^1,2,3,4, Hong Zhang^6,5, Masakazu Agetsuma^7,8, Junichi Nabekura^7,4, Kohei Otomo^2,3,9, Yosuke Okamura^5,10,11, Tomomi Nemoto^2,3,4 (1. Department of Medical and Robotic Engineering Design, Faculty of Advanced Engineering, Tokyo University of Science, 2. Biophotonics Research Group, Exploratory Research Center on Life and Living Systems (ExCELLS), National Institutes of Natural Sciences, 3. Division of Biophotonics, National Institute for Physiological Sciences, National Institutes of Natural Sciences, 4. School of Life Science, The Graduate University for Advanced Studies (SOKENDAI), 5. Micro/Nano Technology Center, Tokai University, 6. School of Chemical Engineering and Technology, Tianjin University, 7. Division of Homeostatic Development, National Institute for Physiological Sciences, National Institutes of Natural Sciences, 8. Quantum Regenerative and Biomedical Engineering Team, Institute for Quantum Life Science, National Institutes for Quantum Science and Technology (QST), 9. Department of Biochemistry and Systems Biomedicine, Graduate School of Medicine, Juntendo University, 10. Department of Applied Chemistry, School of Engineering, Tokai University, 11. Course of Applied Science, Graduate School of Engineering, Tokai University)

Keywords:Two-photon microscopy, Cranial window, Nanosheet, Mouse brain