[2P] 81 Imaging and Visualization

[2P-485] Whole-brain expansion microscopy coupled with 3D immunostaining by CUBIC-HV2.0

*Reo Kurusu¹, Kohei Otomo^1,2,3,4,5, Etsuo A Susaki^1,2,3 (1. Biochemistry II, Juntendo University School of Medicine, 2-1-1 Hongo, Bunkyo-ku, Tokyo, 113-8421, Japan, 2. Department of Biochemistry and Systems Biomedicine, Juntendo University Graduate School of Medicine, 2-1-1 Hongo, Bunkyo-ku, Tokyo, 113-8421, Japan, 3. Nakatani Biomedical Spatialomics Hub, Juntendo University Graduate School of Medicine, 2-1-1 Hongo, Bunkyo-ku, Tokyo, 113-8421, Japan, 4. Division of Biophotonics, National Institute for Physiological Sciences, National Institutes of Natural Sciences, 5-1 Higashiyama, Okazaki, Aichi, 444-8787, Japan, 5. Biophotonics Research Group, Exploratory Research Center for Life and Living Systems, National Institutes of Natural Sciences, 5-1 Higashiyama, Okazaki, Aichi, 444-8787, Japan)

Keywords:Tissue clearing, 3D immunostaining, expansion microscopy, light-sheet microscopy