Exhibitors' information
[02] Funakoshi Co., Ltd.
Thank you for visiting our Funakoshi booth!
Funakoshi Co., Ltd. (Tokyo, Japan) offers unique products for neuroscience research.These products will accelerate your research and help to reveal new findings!
This novel dye succeeded in observation of dynamic biosynthesis, degradation and movement of LDs in live cells.
Ratiometric imaging of neuronal cells
Primary cultured hippocampal neurons (DIV 3 or DIV 12) from E17.5 mice were stained with 300 nM LipiORDER in HBSS for 10 min and observed by confocal laser microscopy (Ex. 405 nm, Em. 470-550 nm for Green channel and >550 nm for Red channel). Ratiometric analysis was performed with ImageJ using green and red channel data and lipid order was shown by green-to-red pseudocolor (Lo■■■■■■■Ld).
AcroleinRED specifically react with either extracellular acrolein conjugate released from cell surface lipids or intracellular acrolein generated via enzymatic pathway and label acrolein with TAMRA fluorophore.
Observation of oxidative stress-induced acrolein production
HUVECs were pretreated with 0-1000 μM H2O2 for 2 hours and subsequently treated with 10 μM AcroleinRED for 30 min. Right after labeling, cells were washed, stained with hoechest and observed under live cell condition. In the absence of H2O2, the acrolein endogenously produced by HUVECs was observed. Intracellular TAMRA signals were increased in H2O2 dose-dependent manner compared with the endogenous acrolein level.
Phone : +81-3-5684-6296
Fax : +81-3-5684-6297
Email: export@funakoshi.co.jp
*Please contact your local distributors for orders and quote request.
Funakoshi Co., Ltd. (Tokyo, Japan) offers unique products for neuroscience research.These products will accelerate your research and help to reveal new findings!
LipiDye II
-Live imaging fluorescent dye for lipid droplets (LDs)-
LipiDye II is a high sensitive, low cytotoxic and super-photostable fluorescent dye for Lipid Droplets (LDs). LipiDye II enables to detect very small LDs (less than 1µm) and to perform long-term time-lapse imaging, including Z-stack imaging.-Live imaging fluorescent dye for lipid droplets (LDs)-
This novel dye succeeded in observation of dynamic biosynthesis, degradation and movement of LDs in live cells.
LipiORDER
-Membrane Lipid Order Imaging Dye-
LipiORDER is a photostable imaging dye for membrane lipid order (Lo/Ld). LipiORDER is excited at around 400 nm wavelength, which is compatible with live-cell imaging and changes its emission fluorescent color from green to red depending on membrane lipid order.-Membrane Lipid Order Imaging Dye-
Ratiometric imaging of neuronal cells
Primary cultured hippocampal neurons (DIV 3 or DIV 12) from E17.5 mice were stained with 300 nM LipiORDER in HBSS for 10 min and observed by confocal laser microscopy (Ex. 405 nm, Em. 470-550 nm for Green channel and >550 nm for Red channel). Ratiometric analysis was performed with ImageJ using green and red channel data and lipid order was shown by green-to-red pseudocolor (Lo■■■■■■■Ld).
AcroleinRED
-Acrolein Detection Reagent in Live Cells-
Our AcroleinRED is the world first cell-based acrolein-detection reagent in live cells condition without any pre-treatment and cell lysis.-Acrolein Detection Reagent in Live Cells-
AcroleinRED specifically react with either extracellular acrolein conjugate released from cell surface lipids or intracellular acrolein generated via enzymatic pathway and label acrolein with TAMRA fluorophore.
Observation of oxidative stress-induced acrolein production
HUVECs were pretreated with 0-1000 μM H2O2 for 2 hours and subsequently treated with 10 μM AcroleinRED for 30 min. Right after labeling, cells were washed, stained with hoechest and observed under live cell condition. In the absence of H2O2, the acrolein endogenously produced by HUVECs was observed. Intracellular TAMRA signals were increased in H2O2 dose-dependent manner compared with the endogenous acrolein level.
Contact information
Funakoshi Co., Ltd.Phone : +81-3-5684-6296
Fax : +81-3-5684-6297
Email: export@funakoshi.co.jp
*Please contact your local distributors for orders and quote request.
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Department
Export business
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Address
113-0033
9-7 Hongo 2-Chome, Bunkyo-ku, Tokyo 113-0033 JAPAN -
Tel
03-5684-1647
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Web site, SNS
