The 44th Annual Meeting of the Japan Neuroscience Society / The 1st CJK International Meeting

Exhibitors' information

[02] Funakoshi Co., Ltd.

Funakoshi Co., Ltd.

Thank you for visiting our Funakoshi booth!
Funakoshi Co., Ltd. (Tokyo, Japan) offers unique products for neuroscience research.These products will accelerate your research and help to reveal new findings!
 
LipiDye II
 -Live imaging fluorescent dye for lipid droplets (LDs)-
LipiDye II is a high sensitive, low cytotoxic and super-photostable fluorescent dye for Lipid Droplets (LDs). LipiDye II enables to detect very small LDs (less than 1µm) and to perform long-term time-lapse imaging, including Z-stack imaging.
This novel dye succeeded in observation of dynamic biosynthesis, degradation and movement of LDs in live cells.

 
   LipiDye II
LipiORDER
 -Membrane Lipid Order Imaging Dye-
LipiORDER is a photostable imaging dye for membrane lipid order (Lo/Ld). LipiORDER is excited at around 400 nm wavelength, which is compatible with live-cell imaging and changes its emission fluorescent color from green to red depending on membrane lipid order.

   LipiORDER data
Ratiometric imaging of neuronal cells
Primary cultured hippocampal neurons (DIV 3 or DIV 12) from E17.5 mice were stained with 300 nM LipiORDER in HBSS for 10 min and observed by confocal laser microscopy (Ex. 405 nm, Em. 470-550 nm for Green channel and >550 nm for Red channel). Ratiometric analysis was performed with ImageJ using green and red channel data and lipid order was shown by green-to-red pseudocolor (LoLd).
 
AcroleinRED
 -Acrolein Detection Reagent in Live Cells-
Our AcroleinRED is the world first cell-based acrolein-detection reagent in live cells condition without any pre-treatment and cell lysis.
AcroleinRED specifically react with either extracellular acrolein conjugate released from cell surface lipids or intracellular acrolein generated via enzymatic pathway and label acrolein with TAMRA fluorophore.

 AcroleinRED
Observation of oxidative stress-induced acrolein production
HUVECs were pretreated with 0-1000 μM H2O2 for 2 hours and subsequently treated with 10 μM AcroleinRED for 30 min. Right after labeling, cells were washed, stained with hoechest and observed under live cell condition. In the absence of H2O2, the acrolein endogenously produced by HUVECs was observed. Intracellular TAMRA signals were increased in H2O2 dose-dependent manner compared with the endogenous acrolein level.
 
Contact information 
 Funakoshi Co., Ltd.
 Phone : +81-3-5684-6296
 Fax : +81-3-5684-6297
 Email: export@funakoshi.co.jp

*Please contact your local distributors for orders and quote request.