Session 5

[BISC5-04(Invited)] Enhanced High-Speed Three-Photon Fluorescence Imaging by Optimized Cr:Forsterite Laser

*Shih-Hsuan Chia¹, Chen Chi-Wen¹, Je-Chi Jang¹ (1. National Yang Ming Chiao Tung University, Taiwan)

This manuscript reports on the utilization of a Cr:Forsterite laser for high-speed three-photon fluorescence imaging in transgenic fruit flies. Through this approach, we achieved a remarkable tenfold increase in signal-to-background ratio at various depths, facilitating whole brain imaging in comparison to conventional two-photon methods.