BISC Poster Session

[BISCp-01] Scanless three-dimensional photostimulation with single cell resolution using time-multiplexed multi-line temporal focusing

*Kenta Inazawa^1,2,3, Keisuke Isobe^1,2, Takayuki Michikawa^1,4, Kana Namiki⁴, Atsushi Miyawaki^1,4, Katsumi Midorikawa¹ (1. RIKEN RAP, 2. Graduated of Biostudies, Kyoto University, 3. Hamamatsu K.K., 4. RIKEN CBS)

We developed a scanless three-dimensional patterned illumination system that combines time-multiplexed multi-line temporal focusing with the CGH technique. In our system, even the 1/e² width of axial resolution is 5.3 μm, which is better than single cell size. We also demonstrated precise scanless excitation in 3D by suppressing hologram distortion and multi-spot interference.