[BISCp-01] Scanless three-dimensional photostimulation with single cell resolution using time-multiplexed multi-line temporal focusing
We developed a scanless three-dimensional patterned illumination system that combines time-multiplexed multi-line temporal focusing with the CGH technique. In our system, even the 1/e2 width of axial resolution is 5.3 μm, which is better than single cell size. We also demonstrated precise scanless excitation in 3D by suppressing hologram distortion and multi-spot interference.
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