Imaging / Lighting 3

[LDC13-02(Invited)] All-pulsed two-photon STED microscopy for nanoscale tissue imaging

*Hirokazu Ishii^1,2, Kohei Otomo³, Tomomi Nemoto^1,2 (1. Exploratory Research Center on Life and Living Systems (ExCELLS), National Institute of Natural Sciences, 2. National Institute for Physiological Sciences (NIPS), National Institute of Natural Sciences, 3. Graduate School of Medicine, Juntendo University)

We developed a two-photon excitation STED microscope utilizing laser-diode-based pulsed light sources and time-gated fluorescence detection, namely, all-pulsed 2PE-gSTED microscopy. Time-gating effectively eliminated undesired signals from brain tissue, resulting in the spatial resolution of 123.3 ± 7.1 nm for the 2PE-gSTED images, which was 1.4 times higher than that for the non-gated images.