[1P-10*] Structure analysis of chignolin and its mutant by NMR
Chignolin, which was designed by Honda et al., is an artificial mini-protein consisting of 10 amino acids:GYDPETGTWG. Its atomic coordinates were determined by NMR. It is widely used to test new simulation algorithm and analysis methods. Chignolin is characterized by two stablestates in molecular dynamics (MD) simulations: a native state and a misfolded state. In our previous works, we proposed several mutations of Thr8 to stabilize the misfolded structure rather than the native structure. Here, we show the results of determination of structures of super-chignolin, chignolin and its mutant by NMR analysis.