[2P-1] Poster 2 (2P-01ー2P-37)

[2P-37(YSA-2)] Comprehensive interaction analysis of the SRK/SP11 complex structures that control self/nonself-discrimination mechanism in Brassica

^○Yoshitaka Moriwaki¹, Tohru Terada¹, Kohji Murase², Seiji Takayama², Kentaro Shimizu¹ (1.Dept. of Biotechnol., Grad. Sch. of Agri. and Life Sci., Univ. of Tokyo, 2.Dept. of Appl. Biol. Chem., Grad. Sch. of Agri. and Life Sci., Univ. of Tokyo)

Self-incompatibility (SI) is one of the most important systems to prevent self-fertilization and thereby promote genetic diversity within a species. The self/nonself-discrimination of SI is controlled by a single polymorphic locus, called S-locus, and S-locus receptor kinase (SRK) in pistil and S-locus protein 11 (SP11) in pollen are responsible for SI in Brassica flowers. If both proteins are derived from the same S-locus, the pollen will be rejected to prevent self-pollination. Although two S-haplotypes of their complex crystal structures have been determined in recent years, a comprehensive analysis of their interactions is still challenging because of the sequential and structural diversity of SP11 molecule and the presence of over 100 S-haplotypes. Here we present a computational modeling method of highly reliable SP11 and SRK/SP11 complex structures and comprehensive interaction analysis between SRK and SP11 molecules using MD simulations. Our method will also contribute to future analysis to identify unknown pairs of SRK-like receptors and SP11-like ligands even in other flowers than Brassica.