[3P-55] 細胞内の時空間制御とMinシステムが細胞サイズの空間で創発するタンパク質の波とその相の分子メカニズム
Min system is a bacterial spatiotemporal regulation system for cell division. Three remarkable features of the Min system are i) a reaction-diffusion coupling of molecules (MinDE) shapes spatiotemporal organization (Min wave), ii) Conformational dynamics of MinE is a key to emerge the Min wave, and iii) Min wave appears at limited concentration ranges of MinDE. In this study, we report reconstitution of Min wave in cell-sized space by the finding that a protein crowding environment is required for the reconstitution due to large surface-area-to-volume ratio. By using the reconstituted system, we found suppression of spontaneous localization of MinE by protein crowder was the trigger for the emergence of Min wave in cell-sized space and revealed that the equilibrium of MinE conformational dynamics was a regulator of the limited concentration ranges of MinE for Min wave emergence. We also showed that spatiotemporal regulation of Min system was able to be controlled by a reconstituted transcription-translation system in artificial cells. The findings in this study shed light on the molecular mechanism of spatiotemporal regulation of proteins that emerged in cell-sized, and contribute to design principles for self-assembly molecules.