[WS4] APPA/PSSJワークショップ 蛋白質研究の世界連携に向けて

[WS4-09] DEVELOPMENT OF NOVEL BACTERIAL CONSORTIUM FOR BIODEGRADATION OF PARAFFIN WAX IN CRUDE OIL

^○Raja Noor Zaliha Raja Abdul Rahman^1,2, Nur Aina Adlan¹, Malihe Masomian^1,4, Suriana Sabri^1,2, Mohd Shukuri Mohamad Ali^1,3 (1.Enzyme and Microbial Technology Research Centre, Faculty of Biotechnology and Biomolecular Science, Universiti Putra Malaysia, 2.Department of Microbiology, Faculty of Biotechnology and Biomolecular Science, Universiti Putra Malaysia, 3.Department of Biochemistry, Faculty of Biotechnology and Biomolecular Science, Universiti Putra Malaysia, 4.Centre for Virus and Vaccine Research, School of Science and Technology, Sunway University)

The deposition of paraffin wax in crude oil is a problem in the oil and gas industry due to the high cost of controlling wax deposition in the pipeline. As an alternative to prevent wax deposition, this study aimed to develop a novel bacterial consortium for the biodegradation of paraffin wax in crude oil. The 11 paraffin wax degrading bacteria screened at 70 °C using crude oil B can degrade the oil and showed better growth, and degradation when yeast extract medium was used. Molecular identification showed that the isolates are Geobacillus kaustophilus strain N3A7, NFA23, DFY1, Parageobacillus caldoxylosilyticus strain DFY3 and AZ72, Geobacillus jurassicus strain MK7, Anoxybacillus geothermalis strain D9 (previously identified), Geobacillus thermocatenulatus strain T7, and Geobacillus stearothermophilus strain SA36, AD24, and AD11. Gas chromatography-mass spectrometry (GCMS) analysis showed more than 70% biodegradation efficiency by strains AD11, DFY1, AD24, N3A7, and MK7 with higher ADH, lipase, and esterase activities compared to other strains. Among the five consortia developed based on the biodegradation efficiency of 11 bacterial strains, consortium 3 (77.8%) showed the highest biodegradation, more long-chain alkane degraded, and higher AMO, ADH, lipase, and esterase activities compared to other consortia. The degradation of long-chain alkane (paraffin wax) by all consortia also caused an increase of short-chain alkanes contents. Bacteria such as P. caldoxylosilyticus strain DFY3, P. caldoxylosilyticus strain AZ72, and G. kaustophilus strain N3A7 were dominant in consortium 1, consortium 2, and consortium 3, respectively based on the denaturing gradient gel electrophoresis (DGGE) results. The bacteria in consortium 4 and consortium 5 both showed dominance on different days of incubation. In conclusion, the degradation of more long-chain hydrocarbon and the increase of some short-chain hydrocarbons by bacterial consortia could improve oil viscosity and reduce oil production loss.