日本分子生物学会/日本生化学会

16:45 〜 18:45

[1LBA085] Temporal Analysis of Flooded Soybean Root Exposed to Silver Nanoparticles using Proteomic Technique

〇Ghazala Mustafa1,2、Katsumi Sakata3、Setsuko Komatsu1,2 (1.Univ. Tsukuba、2.NARO Inst. Crop Sci.、3.Maebashi Inst. Technol.)

soybean, flooding, proteomics, nanoparticles, energy metabolism

Flooding negatively affects the soybean growth; however, silver nanoparticles enhanced the growth under stress. To study the effects of silver nanoparticles on soybean under flooding stress in time-course manner, a gel-free proteomic technique was used. The morphological analysis of early-stage soybean exposed to flooding with silver nanoparticles of various sizes and concentrations revealed enhanced seedling growth by treatment with 15 nm silver nanoparticles at 2 ppm concentration. Differentially changed 107 root proteins were predominantly associated with stress, signaling, and cell metabolism. Hierarchical clustering divided these proteins into 3 clusters. Based on cluster analysis, the abundances of glyoxalase II 3 and fermentation related proteins, which were pyruvate decarboxylase 2 and alcohol dehydrogenase 1, were time-dependently increased under flooding stress, but decreased in response to silver nanoparticles. Six enzymes involved in metabolic pathways were analyzed at the transcriptional level. The alcohol dehydrogenase 1 and pyruvate decarboxylase 2 genes were up-regulated under flooding stress while down-regulated in response to silver nanoparticles. Moreover, comparatively low transcript level of glyoxalase II 3 under silver nanoparticles treatment implies that less cytotoxic by-products of glycolysis are produced in silver nanoparticles exposed soybeans as compared to flooded soybean. These results suggest that the silver nanoparticles treated soybeans have experienced less oxygen-deprivation stress, which might be the key factor for better growth performance of silver nanoparticles treated soybeans under flooding stress.