16:45 〜 18:45
[1LBA088] Omics Analysis of Leaf from Clematis terniflora DC. under Binary Stress
Omics, UV-B stress, plant leaf
Clematis terniflora DC. is a valuable resource for anti-inflammatory activity and UV-B irradiation can change this effect. To understand the systematic response mechanism in plant under UV-B stress, transcriptomics, proteomics, and metabolomics were performed. Leaves were collected from UV-B treated plants without or with dark. Untreated plants were used as control. Genes related to photosynthesis, terpenoids/polyketedes metabolism, cofactors/vitamins metabolism, and ether lipid metabolism were down regulated. However, genes related to steroid hormone biosynthesis, glycan biosynthesis/metabolism, betalain biosynthesis, and signaling molecules/interaction were up regulated. Metabolites related to carbohydrate metabolism, lipid metabolism, secondary metabolism, and cofactors/vitamins metabolism were decreased. However, metabolites related to amino acid biosynthesis, tricarboxylic acid circle, and oxidative phosphorylation were increased. Furthermore, the tendencies of protein abundance were almost same as those of gene expression. These results suggest that Clematis terniflora DC. might respond to the binary stress by decreasing carbon assimilation and increasing secondary metabolites.