日本分子生物学会/日本生化学会

16:45 〜 18:45

[2LBA037] Biochemical changes of salivary gland adenoid cystic carcinoma cells induced by SGI-1776

〇Xin Zhu1、Yizheng Wu3、Zhiqiang Ling1、Minghua Ge2 (1.Zhejiang Cancer Research Institute, Zhejiang Cancer Hospital、2.Department of Head and Neck Surgery, Zhejiang Cancer Hospital、3.Department of clinical medicine, Zhejiang University School of Medicine)

Salivary adenoid cystic carcinoma, Pim-1, SGI-1776

Background As a kinase, Pim-1 is proved to phosphorylate a series of substrates to exert its founction and play important role in many malignancies by elevated expression. It is warranted that depressing of Pim-1 activity might be a neoteric oncological treatment strategy. SGI-1776 is a novel small molecule and the first clinically tested inhibitor of Pim kinase family. The effect of SGI-1776 on salivary adenoid cystic carcinoma (ACC) was investigated in the present study. Methods Expressions of Pim-1 in ACC and adjacent normal tissues were measured by Westernblot. After SACC-83 and SACC-LM cells were exposed to SGI-1776, cell proliferation was analyzed by BrdU assay and colony formation. Cell cycle, apoptosis, and mitochondrial membrane potential were detected by flow cytometry. Caspase-3 activity was measured by Fluorescence microplate reader. Effects of Pim-1 on cells invasion ability were evaluated by scratch assay.Results Pim-1 highly expressed in ACC tissue compared with adjacent normal tissues. SGI-1776 reduces cell proliferation, induces apoptosis, causes cell cycle arrest, mitochondrial depolarization, decreases invasive ability and increases caspase-3 activity in SACC-83 and SACC-LM cells.Conclusion This study confirmed the pivotal role of Pim-1 in ACC. It is also deduced that targeting Pim-1 kinase signal pathway by SGI-1776 would be a promising therapeutic method for ACC.