4:45 PM - 6:45 PM

[3P0548] Construction of a high density mutant library in soybean and development of screening system by next generation sequencing

〇Akito Kaga¹, Mai Tsuda^1,2, Toyoaki Anai³, Takehiko Shimizu¹, Takashi Sayama¹, Kyoko Takagi^1,6, Kayo Machita¹, Satoshi Watanabe^1,3, Minoru Nishimura^1,4, Naohiro Yamada⁵, Satomi Mori¹, Harumi Sasaki¹, Hiroyuki Kanamori¹, Yuichi Katayose¹, Masao Ishimoto¹ (1.NIAS, 2.Tsukuba University, 3.Saga University, 4.Niigata University, 5.Nagano vegetable and ornamental crops exp. stn., 6.NARO/ARC)

mutant library, EMS mutagenesis, amplicon sequencing, Nextera technology, DNA pooling