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[2P-362] Multiple site-specific installations of Nε-monomethyl-L-lysine into histone proteins using the cell-based and cell-free protein synthesis systems from release factor 1 (RF1)-knockout Escherichia coli

Yanagisawa Tatsuo1,2, Takahashi Mihoko1,3, Mukai Takahito1,3, Sato Shin1,3, Wakamori Masatoshi1,3, Shirouzu Mikako1,3, Sakamoto Kensaku1,3, Umehara Takashi1,3,4, Yokoyama Shigeyuki1,2 (1.RIKEN SSBC, 2.RIKEN Struct.Biol.Lab., 3.RIKEN CLST, 4.PRESTO, JST)

非天然型アミノ酸, tRNA, 翻訳後修飾, アミノアシルtRNA合成酵素, タンパク質工学

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