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[4P-030] (4T13a-13)Markedly improved protein-protein interaction assay FlimPIA by the mutagenesis of reaction-dependent luciferase conformational change

Ohmuro Yuki1, Kurihara Makoto2, Yamashita Takahiro2,Ueda Hiroshi3 (1.Dept. of Applied Chem., Grad. Sch. of Eng., Kobe Univ., 2.Dept. of Chem. Biotechnol., Sch. of Eng., Univ. of Tokyo, 3.Chemical Resources Lab., Titech)

protein protein interaction, firefly luciferase, conformational change, ATP, coenzyme A

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