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[1T11e-05(1P-078)] 3D structure and thermodynamic changes of PET-hydrolyzing enzyme Cut190 induced upon Ca2+ binding

Senga Akane1, Inaba Satomi1,2, Numoto Nobutaka3, Miyanoiri Yohei4, Yamagami Yuri1, Ito Nobutoshi3, Kawai Fusako5, Oda Masayuki1 (1.Grad. Sch. Life Environ. Sci., Kyoto Pref. Univ., 2.JASRI/SPring-8, 3.Med. Res. Inst., Tokyo Med. Dent. Univ., 4.Inst. Protein Res., Osaka Univ., 5.Center Fiber Textile Sci., Kyoto Inst. Technol.)

キーテクノロジー:differential scanning calorimetry, NMR, X-ray crystallography

Ca2+ binding、conformational change、enzymatic activity、thermal stability

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