Knock-in of large DNA fragment(6kb) into zygote genome by using long single-stranded oligodeoxynucleotides (lssODN) and CRISPR/Cas9 by electroporation

Sei Kondo

[2P-255] Knock-in of large DNA fragment(6kb) into zygote genome by using long single-stranded oligodeoxynucleotides (lssODN) and CRISPR/Cas9 by electroporation

^○Sei Kondo¹, Miho Kuwahara¹, Shanqi Fu¹, Satoko Takeuchi², Kenji Ikeda², Takanori Ishikawa¹, Mitsuaki Ono³, Takashi Nishida¹, Satoshi Kubota¹, Takako Hattori¹ (1.Dept. of Biochem. Mol. Dent., Okayama Univ. Grad. Sch. Med. Dent. Pharm. Sci., 2.Dent. Sch. Okayama Univ., 3.Dept. of Mol. Bio. Biochem., Okayama Univ. Grad. Sch. Med. Dent. Pharm. Sci.)

キーテクノロジー：genome editing

long-noncoding RNA(lncRNA)、CRISPR/Cas9、lssODN、electroporation、knock-in

de421016@s.okayama-u.ac.jp

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The 92nd Annual Meeting of JBS

[2P-255] Knock-in of large DNA fragment(6kb) into zygote genome by using long single-stranded oligodeoxynucleotides (lssODN) and CRISPR/Cas9 by electroporation

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