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[2T12m-06] Knock-in of large DNA fragment(6kb) into zygote genome by using long single-stranded oligodeoxynucleotides (lssODN) and CRISPR/Cas9 by electroporation

Sei Kondo1, Miho Kuwahara1, Shanqi Fu1, Satoko Takeuchi2, Kenji Ikeda2, Takanori Ishikawa1, Mitsuaki Ono3, Takashi Nishida1, Satoshi Kubota1, Takako Hattori1 (1.Dept. of Biochem. Mol. Dent., Okayama Univ. Grad. Sch. Med. Dent. Pharm. Sci., 2.Dent. Sch. Okayama Univ., 3.Dept. of Mol. Bio. Biochem., Okayama Univ. Grad. Sch. Med. Dent. Pharm. Sci.)

キーテクノロジー:genome editing

long-noncoding RNA(lncRNA)、CRISPR/Cas9、lssODN、electroporation、knock-in

de421016@s.okayama-u.ac.jp

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