Advances in next-generation sequencing (NGS) technology and the benefits of cost reductions have made it possible to analyze tens of thousands of cells from a single tissue sample to understand individual cell populations and their behavior in diseased tissues. Because single-cell RNA sequencing (scRNA-seq) is very sensitive, careful tissue handling and processing is critical. Manual tissue processing is time consuming and highly variable depending on the skill of the operator. Ideally, automated, gentler processing would adequately ensure viability and integrity and preserve the original sample condition. In addition, it has recently become clear that dissociating tissue at low temperatures preserves cellular integrity, reduces transcriptional artifacts, and retains the original transcriptome of the tissue more accurately. In this session, we will present the features and performance data of the powerful combination of the VIA Extractor™ tissue disaggregator and the new Cold Dissociation Enzyme Kit.