[3P-0597] Practice and technical barrier of intra-embryonic large cassette knock-in by Adeno-Associated Viral vector with Cas9 ribonucleoprotein electroporation.

〇Naoaki Mizuno¹, Eiji Mizutani¹, Hideyuki Sato¹, Mariko Kasai¹, Tomoyuki Yamaguchi¹, Hiromitsu Nakauchi^1,2 (1.Div. of SCT, IMSUT, Univ. of Tokyo, 2.Dept. of Genetics, Stanford Univ. School of Medicine.)

ゲノム編集, CRISPR/Cas9, ノックイン, AAV