Bacterial cytoplasmic expression of cyclic single-chain FV antibodies by using intein-mediated protein ligation and chaperone co-expression

○Chenjiang Liu, Soichiro Yamauchi, Natsuki Fukuda, Takashi Sato, Yoshihiro Kobashigawa, Hiroshi Morioka

[3P-0387] Bacterial cytoplasmic expression of cyclic single-chain FV antibodies by using intein-mediated protein ligation and chaperone co-expression

○Chenjiang Liu¹, Soichiro Yamauchi¹, Natsuki Fukuda¹, Takashi Sato¹, Yoshihiro Kobashigawa¹, Hiroshi Morioka¹ (1.Grad. Sch. Pharm. Sci. Kumamoto Univ.)

scFv, split intein, chaperone, protein cyclization, protein expression

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The 43rd Annual Meeting of the Molecular Biology Society of Japan

[3P-0387] Bacterial cytoplasmic expression of cyclic single-chain FV antibodies by using intein-mediated protein ligation and chaperone co-expression

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