[16p-Z22-1~15] 12.7 Biomedical Engineering and Biochips

[16p-Z22-10] Development of nano-micro integrated substrate for detection of Single-cell secretion by using LSPR

〇Ain Obara^1,2, Yuhei Terada^1,2, Masato Saito^1,2, Hyota Takamatsu³, Eiichi Tamiya^2,4 (1.Graduate school of Engineering, Osaka Univ., 2.AIST PhotoBIO-OIL, 3.Graduate school of Medicine, Osaka Univ., 4.ISIR, Osaka Univ.)

Keywords:LSPR, Single-cell, cytokine

It is important for understanding pathology to assay IL-6 secreted from single-cell comprehensively. Localized surface plasmon resonance (LSPR) is a candidate to detect target molecules in label free and real time. In this study, we developed a substrate integrated LSPR gold capped-nanopillar and micro well structures with two step nanoimprint. In addition, we detected biomolecules by LSPR imaging on a substrate.