[3Ea02-11] SO/BI

[3Ea07S] In Situ Observation of Lipid Bilayer Construction and Cytochrome c Binding on Cytochrome c Oxidase Modified Electrode

*Ryoya Sano¹, Narumi Yoshida¹, Masaru Kato^1,2, Ichizo Yagi^1,2 (1. Graduate School of Environmental Science, Hokkaido University, 2. Faculty of Environmental Earth Science, Hokkaido University)

We prepared cytochrome c oxidase (CcO)-modified gold electrodes through self-assembled monolayers (SAM) of mixed alkanethiols and tracked the surface assembly of bilayer lipid membranes (BLM) and the binding of cytochrome c (Cyt c) to the electrode by time-resolved surface-enhanced infrared absorption (SEIRA) spectroscopy. The SEIRA spectra suggest that terminal functional groups of alkylthiols used for the SAM affected the CcO orientation at the surface and binding amounts of Cyt c to the surface-tethered CcO. Electrochemical measurements of CcO-modified electrodes were performed before and after the Cyt c binding in an oxygen atmosphere. Catalytic cathodic currents, which are associated with the oxygen reduction catalyzed by the CcO, were observed and were increased after the binding of Cyt c.