[P3-3-24] IL-22 promotes β-Defensin 3 production in the oral cavity
キーワード：β-Defensin 3、IL-22、GE1 cell
It has been reported that probiotic Lactic acid bacteria (LAB) are involved in maintaining and regulating homeostasis in the intestinal tract and the whole body by activating the intestinal immune system. We have reported that the administration of LAB enhances the innate immune system and produces β-defensin 3 (bD3) in saliva, suppressing the onset of periodontitis and reducing its pathogenesis using a mouse model of periodontal disease. It is unclear how the activation of the immune mechanism in the intestines affects the production of bD3 in saliva. In this study, we aimed to elucidate the mechanism of induction of bD3 production in gingival epithelial cells by IL-22 stimulation. We analyzed the gene expression of bD3 production induction after IL-22 stimulation in Gingival epithelial cells (GE1) from mice using Real-time PCR, ELISA, and fluorescence-labeled antibody immunostaining. Gene expression and ELISA analysis showed that bD3 increased dependent response to IL-22 stimulation. Similar results were also observed in cultured cells by fluorescence-labeled antibodies. Furthermore, the use of IL-22 neutralizing antibody inhibited the production of bD3, which was confirmed by gene expression and ELISA. The induction of bD3 production by IL-22 stimulation has been reported mainly in the intestinal epithelium, but this study showed that it is also induced in the oral cavity. The present study suggests that the same mechanism may also be applied to other mucosal epithelia where bD3 production may be generated in saliva.