[18p-F306-1~17] 12.7 Biomedical Engineering and Biochips

[18p-F306-3] Pattern size effect in exosome array

〇(PC)Kyohei Okubo¹, Hiromi Kuramochi¹, Akiko Iwaya¹, Shoichi Tsuchiya², Rei Okamura¹, Takanori Ichiki^1,2 (1.The Univ. of Tokyo, 2.iCONM)

Keywords:Single particle analysis, Exosome, Array chip technology

Exosomes, one of extracellular vesicles in size ranging from 30–150 nm in diameter, have recently attracted much attention as promising biomarker for an early stage diagnostic test. Here, we propose an assay platform called exosome array on which exosomes are separately immobilized and analyzed in the similar manner as DNA array. Polyethyleneglycol (PEG)-lipid modified nanodot array is formed on the Si substrate by a combination of electron beam lithography, selective chemical modification, and lift-off process. PEG-lipid derivative has an oleyl group at its end, at which exosomes are attached via hydrophobic interaction. Exosomal suspension derived from leukemia HL60 cells are flown over the array. The topography of the dotarray was observed using atomic force microscope, revealing single exosome was tethered on the array. In presentation, we will discuss the effect of dotarray pattern size on attached exosomes.