2:30 PM - 2:45 PM
[12p-S402-6] Highly sensitive detection of α-Synuclein in serum of Parkinson's disease patients by liposome-based sensor
Keywords:Biosensor, Liposome, Alpha-Synuclein
Alpha-synuclein is the causative agent of Parkinson's disease, and monomers of alpha-synuclein acquire toxicity during the process of aggregation, fibril elongation, and polymerization. Therefore, detection of trace amounts of αSyn polymer is very effective for early diagnosis of Parkinson's disease.
Recently, it has been reported that NaCl promotes the aggregation of monomeric αSyn, but NaCl also affects other proteins that are foreign substances in patient serum, causing noise. Hence, in this study, we investigated a new method for detecting α-synuclein in patient serum using NaCl with higher sensitivity and less susceptibility to adulteration.
A QCM sensor was used, and NaCl 0.5 M was used as a buffer to fill the sensor cell. The serum was centrifuged at a higher speed than before, and the monomer solution was filtered. The serum from PD patients showed a larger decrease in frequency than the control, confirming that patient identification was possible even in NaCl solution.
Recently, it has been reported that NaCl promotes the aggregation of monomeric αSyn, but NaCl also affects other proteins that are foreign substances in patient serum, causing noise. Hence, in this study, we investigated a new method for detecting α-synuclein in patient serum using NaCl with higher sensitivity and less susceptibility to adulteration.
A QCM sensor was used, and NaCl 0.5 M was used as a buffer to fill the sensor cell. The serum was centrifuged at a higher speed than before, and the monomer solution was filtered. The serum from PD patients showed a larger decrease in frequency than the control, confirming that patient identification was possible even in NaCl solution.