[ODP8A] a. 微生物・微生物の産物の応用

[ODP-229] 抗がん薬誘発性神経障害性疼痛モデルにおいて片側に投与されたA型ボツリヌス神経毒素の両側性の鎮痛効果

○山本 由弥子¹，Arief Waskitho²，Huijiao Yan²，Resmi Raju²，Swarna Lakshmi Raman²，美間 健彦¹，後藤 和義¹，横田 憲治³，松下 治¹，松香 芳三² (¹岡山大・院医歯薬・病原細菌，²徳島大・院医歯薬，³岡山大・院保健)

We have previously shown that botulinum toxin type A (BoNT/A) alleviates trigeminal neuropathy induced by infraorbital nerve constriction. In present study, we generated two rat models of chemotherapy-induced peripheral neuropathy, and assessed the analgesic effect of BoNT/A in chemotherapy-induced neuropathy. Sprague-Dawley male rats (120-140 g) were intraperitoneally injected with anticancer medication, cisplatin (2 mg/kg/day) once a day for four days or vincristine sulfate (100 µg/kg/day) in two 5-day cycles with two days break between cycles (10 injections, 1 mg/kg cumulative dose). Administration of cisplatin or vincristine sulfate produced bilateral mechanical allodynia which was measured as decreased head withdrawal threshold (p < 0.05). BoNT/A (10 MLD) or saline was intracutaneously administered on one side at the center of the whisker pad 15 days after the first administration of the anticancer medication. Unilateral BoNT/A administration in the whisker pad area attenuated chemotherapy-induced mechanical allodynia bilaterally. This result suggests that BoNT/A has the potential central effect.