[3P-0208] Study on regioselective coupling enzyme MCE

〇Taichi Ohshiro¹, Masayuki Toyoda¹, Mio Kawaguchi¹, Hiroshi Tomoda¹ (1.Grad. Sch. of Pharm. Sci., Kitasato Univ.)

ConBio2017

[3P-0209] Generation of genetically modified rats with CYP27B1 deficient or VDR mutation by genome editing to reveal molecular mechanism of vitamin D action

〇Miyu Nishikawa¹, Kaori Yasuda¹, Masashi Takamatsu¹, Keisuke Abe¹, Kyohei Horibe¹, Kairi Okamoto¹, Hiroki Mano¹, Kimie Nakagawa², Naoko Tsugawa³, Toshio Okano², Shinichi Ikushiro¹, Toshiyuki Sakaki¹ (1.Fac. of Engineer., Toyama Pref. Univ., 2.Dep. of Hygenic Sci., Kobe Pharm. Univ., 3.Fac. of Health Nutr., Osaka Shoin Women's Univ.)

ConBio2017

[3P-0210] Effects of 25-hydroxyvitanin D3 administration to CYP27B1 knockout rats generated by CPISPR/Cas9-mediated genome editing

〇Kyohei Horibe¹, Miyu Nishikawa¹, Kaori Yasuda¹, Masashi Takamatsu¹, Keisuke Abe¹, Kairi Okamoto¹, Hiroki Mano¹, Kimie Nakagawa², Naoko Tsugawa³, Toshio Okano², Shiniti Ikushiro¹, Toshiyuki Sakaki¹ (1.Fac. of Engineer., Toyama Pref. Univ., 2.Dep. of Hyganic Sci., Kobe Pharm. Univ., 3.Fac. of Health., Osaka Shoin Women's Univ.)

ConBio2017

[3P-0211] Comparative analysis of 25-hydroxyvitamin D3 action between CYP27B1 deficient rats and mutant VDR transgenic rats generated by genome editing system

〇Keisuke Abe¹, Miyu Nishikawa¹, Kaori Yasuda¹, Kyohei Horibe¹, Masashi Takamatsu¹, Xinyang Deng¹, Hiroki Mano¹, Kairi Okamoto¹, Kimie Nakagawa², Naoko Tsugawa³, Toshio Okano², Shinichi Ikushiro¹, Toshiyuki Sakaki¹ (1.Fac. of Engineer., Toyama Pref. Univ., 2.Dep. of Hygenic Sci., Kobe Pharm. Univ., 3.Dep. of Health., Osaka Shoin Women's Univ.)

ConBio2017

[3P-0212] Simultaneous quantitative analysis of multiple vitamin D metabolites in plasma using LC/MS/MS

〇Kaori Yasuda¹, Miyu Nishikawa¹, Kairi Okamoto¹, Masashi Takamatsu¹, Keisuke Abe¹, Kimie Nakagawa², Naoko Tsugawa³, Toshio Okano², Shinichi Ikushiro¹, Toshiyuki Sakaki¹ (1.Fac. of Engineer., Toyama Pref. Univ., 2.Dep. of Hygienic Sci., Kobe Pharm. Univ., 3.Fac. of Health and Neutrition, Osaka Shoin Women's Univ.)

ConBio2017

[3P-0213] Comparative analysis of 25-hydroxyvitamin D3 metabolism between wild-type and CYP24A1-knockout rats

〇Kairi Okamoto¹, Kaori Yasuda¹, Miu Nishikawa¹, Masashi Takamatsu¹, Keisuke Abe¹, Hiroki Mano¹, Kimie Nakagawa², Naoko Tsugawa³, Toshio Okano², Shinichi Ikushiro¹, Toshiyuki Sakaki¹ (1.Fac. of Engineer., Toyama Pref. Univ. , 2.Dep. of Hygenic Sci., Kobe Pharm. Univ., 3.Fac. of Health., Osaka Shoin Women's Univ.)

ConBio2017

[3P-0214] Mechanistic implications for iron-sulfur cluster biosynthesis deduced from genetic and biochemical analysis of the SufBCD complex

〇Eiki Yuda¹, Naoyuki Tanaka¹, Takashi Fujishiro¹, Kei Hirabayashi², Keiichi Fukuyama³, Kei Wada², Yasuhiro Takahashi¹ (1.Div. of Life Sci., Grad. Sch. of Sci. and Eng., Saitama Univ., 2.Dep. of Med. Sci., Univ. of Miyazaki, 3.Div. of Appl. Chem., Grad. Sch. of Eng., Osaka Univ.)

ConBio2017

[3P-0215] Diversity of iron-sulfur cluster biosynthetic machinery : Genetic analysis of chimeric machinery uniquely distributed in Gram-positive bacteria

〇Nao Yokoyama¹, Chihiro Maruyama¹, Chihiro Nonaka¹, Naoyuki Tanaka¹, Tomohisa Kuzuyama², Kei Asai³, Yasuhiro Takahashi¹ (1.Div. of Life Sci., Grad. Sch. of Sci. and Eng., Saitama Univ., 2.Biotech. Res. Ctr., Univ. Tokyo, 3.Dep. of Biosci., Facul. of Life Sci., Tokyo Univ. of Agri.)

ConBio2017

[3P-0216（3AT12-06）] Crystal structures of CYP90B1, a key enzyme in brassinosteriod biosynthesis, reveal regio- and stereo-specific hydroxylation of phytosterol

〇Keisuke Fujiyama¹, Tomoya Hino¹, Jae Lee Hyoung², Masaharu Mizutani², Shingo Nagano¹ (1.Dept. of Chem. and Biotech., Grad. Sch. of Eng., Univ. of Tottori, 2.Func. Phyt., Grad. Sch. of Agr. Sci., Univ. of Kobe)

ConBio2017

[3P-0217] Effect of purification tag or detergents on the hydroxylation activity of integral membrane alkane hydroxylase AlkB

〇Takaki Okamoto¹, Yuuki Miyauchi², Shingo Nagano², Tomoya Hino² (1.Grad. Sch. of Sus. Sci., Tottori Univ. , 2.Dept. Chem. Biotech., Grad. Schl. Eng., Tottori Univ.)

ConBio2017