[6-1130-P] Postharvest/Food Technology and Process Engineering (6th)

[6-1130-P-18] Optimization and Evaluating of Pomegranate Peel Extract by Micro Wet Milling Using Response Surface Methodology

*Rasool Khan Amini¹, Yutaka Kitamura², Mito Kokawa², M. Z. Islam² (1. Graduate School of Life and Environmental Sciences, University of Tsukuba(Japan), 2. Faculty of Life and Environmental Sciences, University of Tsukuba, 1-1-1, Tennoda, Tsukuba, Ibaraki 305-8572, Japan(Japan))

Keywords:Pomegranate peel extract, Antioxidant, Micro wet milling, phenolics, Response surface methodology

Recently, studies on Pomegranate peel discarded as waste has increased due to high phenolics and antioxidant content as well as its antimicrobial activities. In this study, the extraction method for Pomegranate Peel Extract (PPE) was developed and optimized using the Micro Wet Milling System (MWM). Response Surface Methodology (RSM) was used to determine the optimum condition for Phenolics and their Antioxidant and Antimicrobial activities. The effects of solid to solvent ratio (X₁ :10-30%), Ethanol and water ratio (X₂ : 40-80%), feeding rate (X₃ :10-20 mL/min) and rotational speed (X₄ :20-50 rpm) on Particle Size, Antioxidant activities, Total Phenolic Content (TPC), Catechin content, Gallic Acid and Punicalagin were optimized using RSM. Scanning Electron Microscope (SEM) will be used to study the pomegranate peel cell structure disruption with MWM. Results suggest that Micro Wet Milling (MWM) can produce smaller (minimum 9µm) Particle Size and can be used as a new method for pomegranate peel phenolic extraction. The solid to solvent ratio, ethanol percentage, feeding rate, and rotational speed has significant effects on the phenolics content as well as catechin content antioxidant activities. Further study will be conducted for optimization of phenolics and antimicrobial activities of pomegranate peel extract.