4:00 PM - 4:27 PM
[MS3-01] Treatment of focal articular cartilage defect using human iPS cells-derived cartilage
Keywords:軟骨、iPS細胞、再生医療
Articular cartilage locates the end of the bone and shows lubrication. Articular cartilage lacks self-repairing capacity, and the damage often leads to the onset of osteoarthritis. Here, we have focused on human iPS cells to develop a novel treatment for focal articular cartilage defect using human iPS cell-derived cartilage.
We have developed methods to differentiate human iPS cells into chondrocytes and cartilages. But, the problem in the clinical application was the use of Matrigel, which was used as a substrate to support undifferentiated iPS cells. Matrigel is derived from mouse Swarm tumors and cannot be used in clinical trials, and laminin fragment is used as an alternative. However, the laminin fragment is less efficient than Matrigel in differentiating into chondrocytes. One of the reasons is the difference in cell morphology depending on the culture substrate, and we focused on the Hippo-YAP signaling pathway.
In this study, we found that the efficiency of differentiation into chondrocytes was improved by suppressing YAP. The use of a bioreactor enabled us to generate stably a large amount of high-quality human iPS cell-derived cartilage.
We have developed methods to differentiate human iPS cells into chondrocytes and cartilages. But, the problem in the clinical application was the use of Matrigel, which was used as a substrate to support undifferentiated iPS cells. Matrigel is derived from mouse Swarm tumors and cannot be used in clinical trials, and laminin fragment is used as an alternative. However, the laminin fragment is less efficient than Matrigel in differentiating into chondrocytes. One of the reasons is the difference in cell morphology depending on the culture substrate, and we focused on the Hippo-YAP signaling pathway.
In this study, we found that the efficiency of differentiation into chondrocytes was improved by suppressing YAP. The use of a bioreactor enabled us to generate stably a large amount of high-quality human iPS cell-derived cartilage.