Oral

[O2-E-PM1-04] Effectiveness of Leukocyte- and Platelet-Rich Plasma (L-PRP) on the pulpal healing process following tooth replantation in mice

〇Angela Quispe-Salcedo¹, Mauricio Zapata-Sifuentes¹, Taisuke Watanabe¹, Tomoyuki Kawase², Hayato Ohshima¹ (1. Div Anat Cell Biol Hard Tissue, Niigata Univ Grad Sch Med Dent Sci, 2. Div Oral Bioeng, Niigata Univ Grad Sch Med Dent Sci)

Keywords:Leukocyte and Platelet-rich plasma、tooth replantation、pulpal healing

Aim: This study aimed to evaluate the effect of leukocyte- and platelet-rich plasma (L-PRP) on the dental pulp healing process following tooth replantation. Method: Blood was collected from 4-6-week-old mice via tail veins and moved to a tube containing acid citrate dextrose, as anticoagulant, and the sample was processed to be applied in situ . After maxillary first molars of 3-week-old mice were extracted, the alveolar socket was filled with or without 1.5 uL of leukocyte- and PRP (L-PRP) (experimental or control groups [EG or CG]), followed by tooth replantation. Tooth samples were collected from days 1-28 after operation, processed for HE and AZAN staining, and evaluated by immunohistochemistry for Nestin, CD31, PGP 9.5, and Ki-67. Results: Quantitative analysis of Nestin immunostaining showed a positive tendency towards the healing of the pulpal tissue on day 7 in the EG. Hard-tissue deposition was significantly increased in the EG on day 14. The number of proliferative cells in the coronal and root pulp in the EG was higher than that in the CG on day 7, and subsequently that significantly decreased in the root pulp of the EG on day 14. Conclusion: Our data suggest that the treatment with L-PRP positively affected the healing of the afflicted pulpal tissue after tooth replantation, by increasing cell proliferation and promoting newly-formed hard-tissue deposition in the dental pulp.