[P1-2-33] Comparison and Investigation of Antifungal Susceptibility of Candida albicans Clinical Isolates
Keywords:Candida albicans、抗真菌薬感受性、耐性菌
Oral candidiasis is an opportunistic infection caused by Candida species (mainly Candida albicans), which are endemic in the oral cavity. They are known to cause various symptoms such as oral pain and dysgeusia. Mostly, candidiasis is treated with antifungal drugs for oral administration, rinsing, and application to localized areas. Recently, it is known that the number of antifungal drug-resistant strains has increased, but there have been few reports on the antifungal susceptibility of clinical isolates of Candida albicans in Hokkaido.
In this study, we examined the antifungal susceptibilities of 58 clinical isolates of C. albicans at the Center for Dental Clinics of Hokkaido University Hospital. Six antifungal drugs were used: micafungin, amphotericin B, flucytosine, fluconazole, itraconazole, and voriconazole. Drug susceptibilities were measured by the M27-A3 broth microdilution method recommended by the National Committee for Clinical Laboratory Standards (NCCLS). No strains were resistant to micafungin, amphotericin B, and voriconazole. One resistant strain each to flucytosine, fluconazole, and itraconazole was detected.
These results suggest that antifungal drug-resistant C. albicans in Hokkaido University Hospital is very rare at this moment. However, many clinical isolates were less susceptible to antifungal drugs than IFM40009, the standard strain of C. albicans, and the resistance gene of C. albicans is induced by exposure to antifungal drugs in some cases, suggesting that the number of resistant strains is possible to increase.
Conflict of Interest: The authors declare no conflict of interest.
In this study, we examined the antifungal susceptibilities of 58 clinical isolates of C. albicans at the Center for Dental Clinics of Hokkaido University Hospital. Six antifungal drugs were used: micafungin, amphotericin B, flucytosine, fluconazole, itraconazole, and voriconazole. Drug susceptibilities were measured by the M27-A3 broth microdilution method recommended by the National Committee for Clinical Laboratory Standards (NCCLS). No strains were resistant to micafungin, amphotericin B, and voriconazole. One resistant strain each to flucytosine, fluconazole, and itraconazole was detected.
These results suggest that antifungal drug-resistant C. albicans in Hokkaido University Hospital is very rare at this moment. However, many clinical isolates were less susceptible to antifungal drugs than IFM40009, the standard strain of C. albicans, and the resistance gene of C. albicans is induced by exposure to antifungal drugs in some cases, suggesting that the number of resistant strains is possible to increase.
Conflict of Interest: The authors declare no conflict of interest.