[P1-2-52] Screening and analysis of human oral bacteria that inhibit the growth of periodontal pathogen
Keywords:微生物、バイオフィルム、細菌
More than 700 species of microorganisms are present in dental plaque in the oral cavity, forming the well-balanced oral microbiome. However, dysbiosis of the oral microbiome triggers the inflammatory disease such as periodontitis in the periodontal tissue. In fact, a periodontal pathogen Porphyromonas gingivalis, a major pathogenic bacterium of periodontal disease, is frequently isolated from the active site of periodontal patients. Oral microbiota make various symbiotic relationships with microorganisms. We hypothesize that oral microbiota of healthy human contains specific bacteria that exhibit competition against P. gingivalis. The aim of this study is to screen and identify the candidate bacteria derived from dental plaque of healthy human to inhibit the growth of P. gingivalis strain W83. From bacteria collected from plaque of healthy individuals, we screened for bacteria that inhibit the growth of P. gingivalis. We found that bacteria isolated from dental plaque, which are early colonizer of dental plaque, inhibited the growth of P. gingivalis. In this presentation, we report on the characteristics of the isolated bacteria, including Gram staining and 16S rRNA analysis, growth inhibitory activity against periodontal bacteria including P. gingivalis, and also an attempt to identify antimicrobial factors produced by the isolated bacteria.