[P1-3-21] Effect of mechanical stress on epidermal growth factor receptor (EGFR) in human gingival epithelial cells
Keywords:メカニカルストレス、ヒト歯肉上皮細胞、上皮増殖因子受容体 (EGFR)
To determine the effect of mechanical stretching on epidermal growth factor receptor (EGFR) activation in the human gingival epithelial cell line Ca9-22. Ca9-22 cells were seeded in type IV collagen-coated 2.25 cm2 silica chambers and mechanical loading was applied to the chambers using a stretching device (C S-1700: Strex) at a 20% stretch rate. Samples were prepared immediately after the end of stretching and phosphorylation of EGFR and Focal adhesion kinase (FAK) was examined by Western blotting. FAK was found to be phosphorylated and activated in the experimental group as well as EGFR.In Ca9-22 cells, mechanical stretching induced shedding of membrane proteins, suggesting that the release of various physiologically active substances from the cell membrane may have activated the phosphorylation of EGFR and FAK.