[P1-3-22] Effect of mechanical stress on IL-8 production in human gingival epithelial cells
Keywords:ヒト歯肉上皮細胞、IL-8、メカニカルストレス
Occlusion trauma is the damage to teeth when an excessive force is acted upon them and this may cause inflammatory response or movement of the teeth. As a mechanical stress by injuring gingival and periodontal fibers, occlusal trauma not only effect the alveolar bone, but also periodontium. It causes a wide variety of biological responses. This time we discussed the effect of mechanical stress on IL-8 production in human gingival epithelial cells. After handling of Ca9-22(human gingival epithelial lineage cells) with Type IV collagen coating, spreading at 2.25X105 cells each 2.25cm2 silica chamber. Cell cultures were performed in D-MEM (with 1% or 10%FBS). Chambers underwent continuous cyclic stretch (20% for 30 cycles/min) for 5 days by using stretching appliance (CS-1700, STREX). Stimulation was given once a day for 10min, 50min and 70min. Cells without stretch stimulation were used the control group, which is under the same culture conditions in chambers. Samples were recovered on the fourth and sixth days. The amount of IL-8 in the culture medium was detected by ELISA, and the change of IL-8 mRNA was detected by PCR. IL-8 production was confirmed in the control group of Day4 and Day6. There was no significant difference of IL-8 protein between Day4 experimental and control groups. Moreover, there were also no significant differences of IL-8 mRNA between Day4 experimental and control groups. In the Day6 results, the amount of IL-8 protein in the experimental group was higher than that in the control group. Also, the amount of mRNA was also higher than in the control group.It is possible that 20% mechanical stress may enhance the behavior of human gingival epithelial cells to produce IL-8.