[P2-2-13] The toxicity of composite resin on periodontal ligament fibroblasts
Keywords:コンポジットレジン、歯根膜線維芽細胞、細胞毒性
Currently, the prevalence of root surface caries below the gum line in elderly is increasing. Therefore, the opportunity to use composite resin (CR) in subgingival root surface is increasing. However, it is still unclear the cytotoxicity of CR to periodontal ligament fibroblasts (PDLs).In a preliminary experiment using a direct method, the differences in cytotoxicity were observed among different types of CR in PDLs. In this study, we investigated the toxicity of various CR materials towards PDLs by indirect method using cell culture insert. Beautifil II LS (BFLS: Shofu), Beautifil Flow Plus (BFF: Shofu), Gracefil Bulk Flow (GF: GC), and Clearfil Majesty ES Flow (CF: Kuraray) were used. The toxicity towards human immortalized periodontal ligament fibroblasts (hPDLs) and primary cultured human periodontal ligament fibroblasts (HPLFs) was assessed. The number of viable cells was measured after 48 and 72 hours of incubation with CR. The sample size of CR was 6.5 mm in diameter and 1.5 mm in thickness, following the preparation methods specified in ISO 10993-1,5 and JIS T6001. Co-culture revealed differences in toxicity towards PDLs among the tested materials. In particular, BFLS and BFF exhibited strong cytotoxicity towards PDLs. These CR materials are characterized by the inclusion of surface pre-reacted glass ionomer (SPRG) fillers. When compared to Light Fil II A and Light Fil II P, which do not contain SPRG fillers, the toxicity towards PDLs was reduced. These findings were confirmed in HPLFs. Although the high concentration of the SPRG fillers in the culture medium due to the sample size may have influenced the toxicity, the present findings suggest the cytotoxicity may be caused by SPRG fillers, which release multi-ion components including fluoride, strontium, boron, silicon, aluminum, and sodium.