Poster session

[P2-2-27] Oral and Gut Microbiota in Patients with Autism Spectrum Disorder (ASD)

〇Airi Iida^1,2, Yuki Toyota², Akira Hasebe¹, Yasutaka Yawaka² (1. Dept Oral Mol Microbiol, Hokkaido Univ Grad Sch Dent Med, 2. Dept Dent Child Disabled Person, Hokkaido Univ Grad Sch Dent Med)

Keywords:腸内細菌叢、口腔細菌叢、16SrRNA遺伝子解析、自閉スペクトラム症

Recent studies suggest that gut microbiome play important roles in the pathogenesis of inflammation, which may contribute to ASD pathophysiology. The oral bacteria may be directly or indirectly involved in ASD through the effect on gut microbiome. However, the relationship between oral microbiota and ASD remains unexplored. We identified distinct features of gut and oral microbiota that differed between ASD patients and neurotypical controls by 16SrRNA marker-gene analysis using Miseq (Illumina) and Qiime2 software. We used saliva and stool samples from 10 ASD groups and 10 neurotypical controls groups. A principal coordinate analysis (PCoA) revealed that the bacterial compositions were significantly different by ASD and control groups. The oral microbiota in ASD groups was characterized primarily by Neisseria, Porphyromonas pasteri and Streptococcus. Among gut microbiome obtained from ASD groups, the dominant marker species were characterized by Faecalibacterium, Bacteroides dorei, and Prevotella copri. This study suggest that there could be oral and gut microbial features based on ASD.