Poster session

[P3-2-34] Whole genome analysis of gram-negative filamentous bacilli isolated from saliva.

〇Noriko Shinozaki-Kuwahara¹, Masanori Saito¹, Hiratsuka Koichi², Yoko Tanaka³, Chieko Taguchi⁴, Tomomi Hashizume-Takizawa¹, Ryoki Kobayashi¹, Hidenobu Senpuku¹ (1. Dept Microbiol Immunol, Nihon Univ Sch of Dent at Matsudo, 2. Dept Biochem Mol Biol, Nihon Univ Sch of Dent at Matsudo, 3. Dept Special Needs Dent, Nihon Univ Sch of Dent at Matsudo, 4. Dept Community Oral Health, Nihon Univ Sch of Dent at Matsudo)

Keywords:唾液、口腔微生物、ゲノム

A gram-stain-negative and very long filamentous bacilli, Leptotrichia sp. was isolated from saliva of healthy human. In this study, complete genome sequence of Leptotrichia sp. SK-5^T has determined and analyzed.
De novo assembly of the multiplexed microbial genome data generated by the PacBio RSII sequencer was performed. Genome sequence obtained in this study was annotated using DFAST annotation pipeline. Digital DNA-DNA hybridization (dDDH) and average nucleotide identity (ANI) between SK-5^T and its related species, Leptotrichia hofstadii and Leptotrichia buccalis based on 16S rRNA gene sequences, were calculated, respectively.
The complete genome of strain SK-5^T consisted of 2,536,480 bp in a single circular chromosome with a G+C content of 29.8%. A total of 2,432 CDSs, 15 rRNAs, 46 tRNAs, and 9 CRISPRs. The dDDH and ANI values between strain SK-5^T and its closely related species, L. hofstadii and L. buccalis were 58.0 and 45.0%, and 93.7 and 89.3 %, respectively.
These results showed below the threshold (70% and 95%, respectively) for species delineation, indicating that SK-5^T represents a novel species.