[P3-2-39] Analysis of red pigment produced by Arachnia rubra strain SK-1T
Keywords:pigment、Arachinia rubra、prodigiosin
Arachnia rubra strain SK-1T is a Gram-positive rod that forms red colonies isolated from the oral cavity of healthy individuals and exhibits antibacterial activity against periodontopathogenic bacteria. Since some pigments have antibacterial effects, we analyzed the pigments produced by the strain. Since some pigments have antibacterial effects, the red pigment produced by the strain SK-1T was analyzed. After whole genome gene sequencing, genome function analysis was performed by KEGG to search for pigments produced. The search yielded hits on prodigiosins, which are red pigments produced by Serratia marcescens. Strain SK-1T that formed red colonies on TSY (Tryptic Soy supplemented with Yeast extract) agar was collected and the extracted pigment using methanol was analyzed by LC/MS. As a result, seven different peaks were identified in the chromatogram at a wavelength of 500 nm. For each peak, the molecular formula was calculated by accurate mass using LC-MS/MS. The SciFinder-n database was used to estimate the structure of the obtained molecular formula. Either peak was presumed to be prodigiosins as a result of the search, but no structures were identified that matched the product ion spectra of the LC-MS/MS analysis. These results suggest that the red pigment produced by strain SK-1T may be a new compound of prodigiosins. Prodigiosin is a tripyrrole red pigment biosynthesized by Serratia marcescens and other bacteria and has been shown to exhibit antibacterial, anticancer, cytotoxic, immunosuppressive, and antiproliferative activities. The novel prodigiosins in strain SK-1T are also expected to be effective as a probiotic because of their antimicrobial effect against periodontopathogenic bacteria.
This work was supported by JSPS KAKENHI Grant Number JP20K10299.
This work was supported by JSPS KAKENHI Grant Number JP20K10299.