[A-HW22] Materials transport and nutrient cycles in watersheds; from headwaters to coastal seas

[AHW22-P06] Microbial phase analysis in aquifer of Ryukyu Limestone Zone using next generation sequencer

*Umi Murao¹, Jun Yasumoto¹, Yasumoto Ko³, Mina Hirose², Nanami Mizusawa³, Ryo Iguchi⁴, Bam H.N. Razafindrabe⁵, Syugo Watabe³ (1.University of the Ryukyus, Faculty of Agriculuture Department of Regional Agricultural Engineering, 2.Tropical Tecnology Plus, 3.Kitasato University School of Marine Biosciences, 4.National Institute of Advanced Industrial Science and Technology, 5.University of the Ryukyus, Faculty of Agriculuture Department of Subtropical Agro-Environmental Sciences)

Keywords:groundwater, microbial community analysis, genetic analysis

Clean water and sanitation (Sustainable development Goals (6)) is one of the world's important issues. People use groundwater in many areas as a water source, but various cases of contamination by agricultural activities and substances derived from human and animal excrements have been reported.

On the other hand, genetic analysis techniques using molecular biological methods have been applied to the analysis of microbial phase in the water environment.

In recent years, the method using the latest analysis equipment of next generation Sequencer is established, mainly for microorganisms. In addition, new monitoring indices such as environmental DNA have been developed. It is thought that the microbial phase in groundwater varies depending on the influence of various environmental factors and human activity, but the actual condition is not clear.

The next generation Sequencer is a very powerful tool used to grasp the microbial phase in groundwater. It is thought that the microbial phase can be an important indicator in water resource management of basins in the future.

The purpose of this study is to investigate the microbial species and their coexistence in groundwater in the Ryukyu Limestone area using the next generation sequencer.