[H-CG35] Advanced life support systems with closed bio-ecosystems and thier applications

[HCG35-P03] Identification of proteins found in the process of slow dehydration in a terrestrial cyanobacterium, Nostoc sp. HK-01

*Hiroko Tanaka¹, Nagamine Myu¹, Tomoko Abe¹, Shunta Kimura², Hiroshi Katoh³, Kaori Tomita-Yokotani⁴ (1.School of Science and Engineering, Tokyo Denki University, 2.Graduate School of Agricultural and Life Sciences, The University of Tokyo, 3.Organization for the Promotion of Regional Innovation, Mie University, 4.Faculty of Life and Environmental Sciences, University of Tsukuba)

Keywords:cyanobacteria, desiccation tolerance, stress protein

Nostoc sp. HK-01 could be used for bio-chemical circulation in a closed ecosystem, including space. Because, it is one of terrestrial cyanobacterium having a tolerance to desiccation stress and it has ability of usefulness as a food. We have been exploring macromolecular substances that would be involved in a mechanism for desiccation tolerance in Nostoc sp. HK-01.
In this study, to detect cellular proteins of Nostoc sp. HK-01 during slow dehydration, proteins extracted from the cells in the course of the dehydration were applied to SDS-polyacrylamide gel electrophoresis. In comparison with the wet cells, the abundance of some proteins changed after dehydration. Genes of some of them were identified and functional analyses of them are in progress.